282 Effects of zinc in the progression of squamous cell carcinoma

Zinc is a trace element that requires for more than 2000 transcription factors regulating cell development, differentiation, and growth of many types including cancer cells. Prior studies reported zinc induced apoptosis in cells, such as glioma, bladder, prostate, breast, melanoma. The effects cutaneous squamous carcinoma (SCC) have not been formally assessed. Two SCC lines (A431 HSC-5) were cultured serum free medium with different concentration zinc. TPEN was used to chelate when the tumor cells containing some experiments. (1, 10, 100 μg/dl) significantly inhibits proliferation at 48 hours 72 dose dependent manner (p<0.05). Effects on migration evaluated by scratch assay. Chelation (2μM) delayed wound closure A431 On other hand, addition accelerates HSC-5 compared control condition We also expression MMPs vitro. Cancer utilize matrix metalloproteinases (MMPs) invade surrounding tissues. acts co-factor MMPs, whereas can alter their expression. Our previous study revealed human tissue expressed variety during progression. For instance, MMP9 actinic keratosis, an early region SCC, increased invasive SCC. prostate suppressed supplementation mRNA will be discussed. These results may increase our understanding role